Mitochondrial membrane potential (ΔΨM) is a central intermediate in oxidative energy metabolism. In mammalian cells, glycolysis and oxidative phosphorylation (OXPHOS) pathways provide the majority of cellular ATP. The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. The CCCP treatment was found to significantly reduce ATP content, increase the amount of phosphorylated AMP-activated protein kinase and elevate reactive oxygen species levels in oocytes. The process of mitophagy was first described over a hundred years ago by Margaret Reed Lewis and Warren Harmon Lewis. It often occurs to defective mitochondria following damage or stress. Mitophagy is the selective degradation of mitochondria by autophagy. The dye is well-retained after aldehyde fixation. MitoTracker Red CMXRos is a red-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The Mitochondrial Membrane Potential Assay Kit (II) is a fluorescent assay that detects the mitochondrial membrane potential in living cells. What is mitochondrial membrane potential assay? JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. What does JC-1 reveal about mitochondria?
0 Comments
Leave a Reply. |